« Back
Home /
BRAF Antibody (T400)

BRAF Antibody (T400)

Item no.	ABC-AP7810E
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	BRAF, p94, BRAF1, RAFB1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1
Available
Primary Accession	P15056
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Bio References	Hingorani, S.R., et al., Cancer Res. 63(17):5198-5202 (2003). Lee, J.W., et al., Br. J. Cancer 89(10):1958-1960 (2003). Davies, H., et al., Nature 417(6892):949-954 (2002). Naoki, K., et al., Cancer Res. 62(23):7001-7003 (2002). Stephens, R.M., et al., Mol. Cell. Biol. 12(9):3733-3742 (1992).
Clonality	Polyclonal
Clone/Animal Names	RB13721
Gene ID	673
Gene Name	BRAF (HGNC:1097)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-BRAF Antibody (T400) at 1:500-2000 dilution Lane 1: K562 whole cell lysate Lane 2: Raji whole cell lysate Lane 3: T47D whole cell lysate Lane 4: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:500-2000
Legend image 2	Anti-BRAF Antibody (T400) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:500
Legend image 3	Immunohistochemical analysis of AP7810E on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:100
Legend image 4	Immunohistochemical analysis of AP7810E on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 4	IHC-P
Dilution image 4	1:100
Other Accession	P28028
Predicted	M
Isotype	Rabbit Ig
Calculated MW	84437
Antigen Region	379-408
Antigen Source	HUMAN
Target/Specificity	This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 amino acids from human BRAF.
NCBI Accession	NP_004324.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AP7810E-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 400 ul

available

Delivery expected until 11/6/2025

Express Delivery: Save 14 days

Compare

Add to wishlist

Get an offer