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GAPDH Antibody (N-term)

GAPDH Antibody (N-term)

ArtNr	ABC-AP7873a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GAPDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-
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Primary Accession	P04406
Antigen Type	Synthetic Peptide
Application	WB, IF, IHC-P
Bio Background	GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Bio References	Azam, S., J. Biol. Chem. 283 (45), 30632-30641 (2008) Lu, J., Biosci. Biotechnol. Biochem. 72 (9), 2432-2435 (2008) Zhou, Y., Mol. Cancer Res. 6 (8), 1375-1384 (2008)
Clonality	Polyclonal
Clone/Animal Names	RB16537
Gene ID	2597
Gene Name	GAPDH {ECO:0000303\|PubMed:2987855, ECO:0000312\|HGNC:HGNC:4141}
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	GAPDH Antibody (N-term) (Cat. # AP7873a) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Confocal immunofluorescent analysis of GAPDH Antibody (N-term)(Cat#AP7873a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 2	IF
Dilution image 2	1:10~50
Legend image 3	All lanes : Anti-GAPDH Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Western blot analysis of lysate from A375 cell line, using GAPDH Antibody (N-term)(Cat. #AP7873a). AP7873a was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Type image 4	WB
Dilution image 4	1:500
Legend image 5	Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using GAPDH Antibody (N-term)(Cat. #AP7873a).AP7873a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Type image 5	WB
Dilution image 5	1:1000
Legend image 6	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 6	IHC-P
Dilution image 6	1:10~50
Isotype	Rabbit Ig
Calculated MW	36053
Antigen Region	62-91
Antigen Source	HUMAN
Target/Specificity	This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human GAPDH.
NCBI Accession	NP_001243728.1; NP_002037.2

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