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GAPDH Antibody (N-term)

GAPDH Antibody (N-term)

Item no.	ABC-AP7873a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GAPDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-
Available
Primary Accession	P04406
Antigen Type	Synthetic Peptide
Application	WB, IF, IHC-P
Bio Background	GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Bio References	Azam, S., J. Biol. Chem. 283 (45), 30632-30641 (2008) Lu, J., Biosci. Biotechnol. Biochem. 72 (9), 2432-2435 (2008) Zhou, Y., Mol. Cancer Res. 6 (8), 1375-1384 (2008)
Clonality	Polyclonal
Clone/Animal Names	RB16537
Gene ID	2597
Gene Name	GAPDH {ECO:0000303\|PubMed:2987855, ECO:0000312\|HGNC:HGNC:4141}
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	GAPDH Antibody (N-term) (Cat. # AP7873a) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Confocal immunofluorescent analysis of GAPDH Antibody (N-term)(Cat#AP7873a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 2	IF
Dilution image 2	1:10~50
Legend image 3	All lanes : Anti-GAPDH Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Western blot analysis of lysate from A375 cell line, using GAPDH Antibody (N-term)(Cat. #AP7873a). AP7873a was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Type image 4	WB
Dilution image 4	1:500
Legend image 5	Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using GAPDH Antibody (N-term)(Cat. #AP7873a).AP7873a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Type image 5	WB
Dilution image 5	1:1000
Legend image 6	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 6	IHC-P
Dilution image 6	1:10~50
Isotype	Rabbit Ig
Calculated MW	36053
Antigen Region	62-91
Antigen Source	HUMAN
Target/Specificity	This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the N-terminal region of human GAPDH.
NCBI Accession	NP_001243728.1; NP_002037.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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