Vergleich

EWSR1 Antibody (C-term)

ArtNr ABC-AP8823B
Hersteller Abcepta
Menge 400 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen WB, FC, IF
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products EWSR1, EWS, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein
Lieferbar
Primary Accession
Q01844
Antigen Type
Synthetic Peptide
Application
IF, FC, WB
Bio Background
EWSR1 is a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain.
Bio References
Bhagirath, T., et.al., Genes Chromosomes Cancer 13 (2), 126-132 (1995)
Clonality
Polyclonal
Clone/Animal Names
RB23546
Gene ID
2130
Gene Name
EWSR1
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Fluorescent confocal image of U251 cell stained with EWSR1 Antibody (C-term)(Cat#AP8823b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
Western blot analysis of EWSR1 Antibody (C-term) (Cat. #AP8823b) in A2058 cell line lysates (35ug/lane). EWSR1 (arrow) was detected using the purified Pab.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
EWSR1 Antibody (C-term) (Cat. #AP8823b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3
FC
Dilution image 3
1:10~50
Other Accession
Q61545
Predicted
M
Isotype
Rabbit Ig
Calculated MW
68478
Antigen Region
628-656
Antigen Source
HUMAN
Target/Specificity
This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 628-656 amino acids from the C-terminal region of human EWSR1.
NCBI Accession
NP_001156757.1; NP_001156758.1; NP_001156759.1; NP_005234.1; NP_053733.2

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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