Comparison

EWSR1 Antibody (C-term)

Item no. ABC-AP8823B
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IF
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products EWSR1, EWS, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein
Available
Primary Accession
Q01844
Antigen Type
Synthetic Peptide
Application
IF, FC, WB
Bio Background
EWSR1 is a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain.
Bio References
Bhagirath, T., et.al., Genes Chromosomes Cancer 13 (2), 126-132 (1995)
Clonality
Polyclonal
Clone/Animal Names
RB23546
Gene ID
2130
Gene Name
EWSR1
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Fluorescent confocal image of U251 cell stained with EWSR1 Antibody (C-term)(Cat#AP8823b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
Western blot analysis of EWSR1 Antibody (C-term) (Cat. #AP8823b) in A2058 cell line lysates (35ug/lane). EWSR1 (arrow) was detected using the purified Pab.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
EWSR1 Antibody (C-term) (Cat. #AP8823b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3
FC
Dilution image 3
1:10~50
Other Accession
Q61545
Predicted
M
Isotype
Rabbit Ig
Calculated MW
68478
Antigen Region
628-656
Antigen Source
HUMAN
Target/Specificity
This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 628-656 amino acids from the C-terminal region of human EWSR1.
NCBI Accession
NP_001156757.1; NP_001156758.1; NP_001156759.1; NP_005234.1; NP_053733.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close