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IL10 Antibody (Center)

IL10 Antibody (Center)

ArtNr	ABC-AP9112C
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	IL-10, IL10, Interleukin-10, Cytokine synthesis inhibitory factor, CSIF
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Primary Accession	P22301
Antigen Type	Synthetic Peptide
Application	FC, WB, IHC-P
Bio Background	The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Bio References	Trajkov, D., et.al., Indian J Tuberc 56 (3), 117-131 (2009) Kim, J.M., et.al., J. Immunol. 148 (11), 3618-3623 (1992)
Clonality	Polyclonal
Clone/Animal Names	RB21234
Gene ID	3586
Gene Name	IL10
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of IL10 Antibody (Center) (Cat. #AP9112c) in MDA-MB435 cell line lysates (35ug/lane). IL10 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:10~50
Legend image 3	IL10 Antibody (Center) (Cat. #AP9112c) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	P79338; P03180
Predicted	EBV, Pr
Isotype	Rabbit Ig
Calculated MW	20517
Antigen Region	27-53
Antigen Source	HUMAN
Target/Specificity	This IL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-53 amino acids from the Central region of human IL10.
NCBI Accession	NP_000563.1

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Menge: 400 ul

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