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AMH Antibody (Center)

AMH Antibody (Center)

ArtNr	ABC-AP9940c-ev
Hersteller	Abcepta
Menge	50 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MIF, AMH, MIS, Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance
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Primary Accession	P03971
Antigen Type	Synthetic Peptide
Application	WB, IHC-P, FC
Bio Background	Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
Clonality	Polyclonal
Clone/Animal Names	RB22167
Gene ID	268
Gene Name	AMH
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-AMH Antibody (Center) at 1:1000-1:2000 dilution Lane 1: Human cerebellum lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000-1:2000
Legend image 2	AP9940c staining AMH in human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 2	IHC-P
Dilution image 2	1:25
Legend image 3	Overlay histogram showing SH-SY5Y cells stained with AP9940c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9940c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 3	FC
Dilution image 3	1:25
Isotype	Rabbit IgG
Calculated MW	59195
Antigen Region	424-451
Antigen Source	HUMAN
Target/Specificity	This AMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-451 amino acids of human AMH.
NCBI Accession	NP_000470.2

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