Comparison

AMH Antibody (Center)

Item no. ABC-AP9940c-ev
Manufacturer Abcepta
Amount 50 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IHC-P
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products MIF, AMH, MIS, Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance
Available
Primary Accession
P03971
Antigen Type
Synthetic Peptide
Application
WB, IHC-P, FC
Bio Background
Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
Clonality
Polyclonal
Clone/Animal Names
RB22167
Gene ID
268
Gene Name
AMH
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
All lanes : Anti-AMH Antibody (Center) at 1:1000-1:2000 dilution
Lane 1: Human cerebellum lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: SH-SY5Y whole cell lysate
Lane 5: U-87 MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1
WB
Dilution image 1
1:1000-1:2000
Legend image 2
AP9940c staining AMH in human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 2
IHC-P
Dilution image 2
1:25
Legend image 3
Overlay histogram showing SH-SY5Y cells stained with AP9940c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9940c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 3
FC
Dilution image 3
1:25
Isotype
Rabbit IgG
Calculated MW
59195
Antigen Region
424-451
Antigen Source
HUMAN
Target/Specificity
This AMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-451 amino acids of human AMH.
NCBI Accession
NP_000470.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ul
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close