PUMA Antibody

ArtNr	ABC-ASC10175
Hersteller	Abcepta
Menge	100 ug
Kategorie	Antibodies Cell Biology Cell Status Apoptosis Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Typ	Antibody Primary
Applikationen	WB, IF, ICC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
Isotype	IgG
Citations	Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001;7(3):683-94. Han J, Flemington C, Houghton AB , Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001;98(20):11318-23.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	PUMA Antibody: JFY1,PUMA,JFY-1,Bcl-2-binding component 3,BCL2 binding component 3
Similar products	PUMA, Bcl-2-binding component 3, JFY-1, PUMA Antibody: JFY1, BCL2 binding component 3
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Manufacturer - Category	Primary Antibodies; Mitochondrion
Manufacturer - Targets	BBC3; A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.
Storage Conditions	PUMA antibody can be stored at 4? for three months and -20?, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Bio Background	PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Clonality	Polyclonal
Gene Name	BBC3
Reactivity	H, M
Calculated Molecular Weight	26498
Antigen Source	Homo sapiens
Purification	PUMA Antibody is affinity chromatography purified via peptide column.
Antibody Form	Liquid
Application Notes	PUMA antibody can be used for detection of PUMA by Western blot at 2 µg/mL. Antibody can also be used for immunocytochemstry at 1 µg/mL. For immunofluorescence start at 2 µg/mL.
Positive Control	K562 Cell Lysate; 3T3 Cell Lysate
Buffer	PUMA Antibody is supplied in PBS containing 0.02% sodium azide.

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Menge: 100 ug

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