PUMA Antibody

BBC3; A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.

PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

BBC3

26498

PUMA Antibody is affinity chromatography purified via peptide column.

PUMA antibody can be used for detection of PUMA by Western blot at 2 µg/mL. Antibody can also be used for immunocytochemstry at 1 µg/mL. For immunofluorescence start at 2 µg/mL.

PUMA Antibody is supplied in PBS containing 0.02% sodium azide.