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HIF2 alpha Antibody

HIF2 alpha Antibody

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Format	ATTO 680
Menge	Inquiry
ArtNr	ABC-ASM10130-A680-100
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	Q99814
Reactivity	H, M
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Human HeLa cell lysates showing detection of HIF2 alpha protein using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Cells CoCl treated.
Type image 2	WB
Dilution image 2	1:500
Legend image 3	Immunohistochemistry analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, dermis positive and muscle positive. .
Type image 3	IHC
Legend image 4	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite.
Type image 4	ICC/IF
Dilution image 4	1:100
Other Accession	NP_001421.4
Isotype	IgG1
Target/Specificity	Detects ~100kDa. Specific for HIF2 Alpha.
Immunogen	GST-human EPAS-1 (HIF2alpha) amino acids 535-631 fusion protein
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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ABC-ASM10130-A680-100-datasheet.pdf

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Menge: Inquiry

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Lieferung vsl. bis 06.06.2024

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