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HIF2 alpha Antibody

HIF2 alpha Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 680
Amount	Inquiry
Item no.	ABC-ASM10130-A680-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q99814
Reactivity	H, M
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Human HeLa cell lysates showing detection of HIF2 alpha protein using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Cells CoCl treated.
Type image 2	WB
Dilution image 2	1:500
Legend image 3	Immunohistochemistry analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, dermis positive and muscle positive. .
Type image 3	IHC
Legend image 4	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite.
Type image 4	ICC/IF
Dilution image 4	1:100
Other Accession	NP_001421.4
Isotype	IgG1
Target/Specificity	Detects ~100kDa. Specific for HIF2 Alpha.
Immunogen	GST-human EPAS-1 (HIF2alpha) amino acids 535-631 fusion protein
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10130-A680-100-datasheet.pdf

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Delivery expected until 7/4/2024