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Cav1.2 Antibody

Cav1.2 Antibody

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Format	APC
Menge	Inquiry
ArtNr	ABC-ASM10178-APC-100
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	P15381
Reactivity	H, M, Rat, Ha
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (ASM10178) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Type image 2	IHC
Dilution image 2	1:1000
Legend image 3	Immunoprecipitation analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:200. Courtesy of: Merrie Mosedale.
Type image 3	IP
Legend image 4	Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:1000.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57-47 (ASM10178). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (ASM10178) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
Type image 5	IHC
Dilution image 5	1:1000
Other Accession	NP_001129994.1
Isotype	IgG1
Target/Specificity	Detects ~240kDa (varies with cell background due to glycosylation).
Immunogen	Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10178-APC-100-datasheet.pdf

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Lieferung vsl. bis 04.07.2024

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