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Cav1.2 Antibody

Cav1.2 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	APC
Amount	Inquiry
Item no.	ABC-ASM10178-APC-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P15381
Reactivity	H, M, Rat, Ha
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (ASM10178) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Type image 2	IHC
Dilution image 2	1:1000
Legend image 3	Immunoprecipitation analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:200. Courtesy of: Merrie Mosedale.
Type image 3	IP
Legend image 4	Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:1000.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57-47 (ASM10178). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (ASM10178) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
Type image 5	IHC
Dilution image 5	1:1000
Other Accession	NP_001129994.1
Isotype	IgG1
Target/Specificity	Detects ~240kDa (varies with cell background due to glycosylation).
Immunogen	Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10178-APC-100-datasheet.pdf

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Delivery expected until 7/4/2024