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Protocadherin Gamma C3 Antibody

Protocadherin Gamma C3 Antibody

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Format	ATTO 680
Menge	Inquiry
ArtNr	ABC-ASM10306-A680-100
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	Q91XX1
Reactivity	H, M
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27 (ASM10306). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody (ASM10306) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma C3 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3 showing detection of ~100 kDa Protocadherin Gamma C3 protein using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27 (ASM10306). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody (ASM10306) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_291059.1
Isotype	IgG1
Target/Specificity	Detects ~100kDa.
Immunogen	Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-C3. Human: 97% identity (83/85 amino acids identical). Rat: 97% identity (83/85 amino acids identical). No significant identity with other Gamma-protocadherin proteins.
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10306-A680-100-datasheet.pdf

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Lieferung vsl. bis 04.07.2024

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