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Protocadherin Gamma C3 Antibody

Protocadherin Gamma C3 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 680
Amount	Inquiry
Item no.	ABC-ASM10306-A680-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q91XX1
Reactivity	H, M
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27 (ASM10306). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody (ASM10306) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma C3 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3 showing detection of ~100 kDa Protocadherin Gamma C3 protein using Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody, Clone S174B-27 (ASM10306). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-cadherin-C3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma C3 Monoclonal Antibody (ASM10306) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_291059.1
Isotype	IgG1
Target/Specificity	Detects ~100kDa.
Immunogen	Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-C3. Human: 97% identity (83/85 amino acids identical). Rat: 97% identity (83/85 amino acids identical). No significant identity with other Gamma-protocadherin proteins.
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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ABC-ASM10306-A680-100-datasheet.pdf

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Delivery expected until 6/6/2024