Acrolein Antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (ASM10332). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (ASM10332) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 µM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) Acrolein Antibody. (G, H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

1:50

WB

Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (ASM10332). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (ASM10332) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

IgG1

Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).

-20ºC