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Acrolein Antibody

Acrolein Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	Biotin
Amount	Inquiry
Item no.	ABC-ASM10332-BI-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (ASM10332). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (ASM10332) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 µM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) Acrolein Antibody. (G, H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Type image 1	ICC/IF
Dilution image 1	1:50
Legend image 2	Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein-BSA using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (ASM10332). Lane 1: Molecular Weight Ladder (MW). Lane 2: Acrolein-BSA (0.5 µg). Lane 3: Acrolein-BSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (ASM10332) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (ASM10332). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (ASM10332) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
Type image 3	FC
Isotype	IgG1
Target/Specificity	Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Immunogen	Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10332-BI-100-datasheet.pdf

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Delivery expected until 6/6/2024