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F(ab')2 Anti-Human IgG (H&L) (Fluorescein Conjugated) Pre-Adsorbed Secondary Antibody

Hersteller Abcepta
Kategorie
Typ Antibody Secondary
Specific against other
Menge 1 mg
Host Goat
ArtNr ABC-ASR2086
Konjugat/Tag FITC
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Bio Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Clonality
Polyclonal
Subtitle
Goat Polyclonal, Fluorescein (FITC)
Immunogen
Human IgG whole molecule
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Fluorescein (FITC)
Application Note
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value
2.7 moles Fluorescein (FITC) per mole of IgG F(ab' )2
Target Species
Human
Physical State
Lyophilized
Target Isotype
IgG (H&L)
Host Isotype
IgG F(ab')2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Sodium Azide
Shipping Condition
Ambient
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Mouse, Rabbit, Rat and Sheep  Serum Proteins.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 04.07.2024 

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