Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Secondary |
Specific against |
other |
Amount |
1 mg |
Host |
Goat |
Item no. |
ABC-ASR2086 |
Conjugate/Tag |
FITC |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Bio Background |
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Clonality |
Polyclonal |
Subtitle |
Goat Polyclonal, Fluorescein (FITC) |
Immunogen |
Human IgG whole molecule |
Concentration (mg/ml) |
1.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Fluorescein (FITC) |
Application Note |
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500 |
Buffer |
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
FP Value |
2.7 moles Fluorescein (FITC) per mole of IgG F(ab' )2 |
Target Species |
Human |
Physical State |
Lyophilized |
Target Isotype |
IgG (H&L) |
Host Isotype |
IgG F(ab')2 |
Reconstitution Volume |
1.0 mL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Sodium Azide |
Shipping Condition |
Ambient |
Purity |
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.