Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody

Polyclonal

Dot Blot of Mouse anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed.
Antigen: Rabbit IgG.
Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: n/a.
Secondary antibody: Mouse anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1, 000 for 1 HR at RT.
Block: MB-070 for 1 HR at RT.

Western Blot of Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) 1:1, 000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody 1:40, 000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

1:500 - 1:2, 500

1.0 mg/mL by UV absorbance at 280 nm

ELISA 1:2, 000-1:10, 000; Western Blot 1:500-1:2, 500; Immunochemistry 1:200-1:1, 000

Rabbit

IgG (H&L)

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Wet Ice

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.