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Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody

Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody

Item no.	ABC-ASR3135
Manufacturer	Abcepta
Amount	1 mg
Category	Antibodies Secondary Antibodies
Type	Antibody Secondary
Specific against	other
Host	Mouse
Conjugate/Tag	AP
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Clonality	Polyclonal
Subtitle	Mouse Polyclonal, Alkaline Phosphatase (Calf Intestine)
Legend image 1	Dot Blot of Mouse anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed. Antigen: Rabbit IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Mouse anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1, 000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.
Type image 1	DB
Legend image 2	Western Blot of Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) 1:1, 000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody 1:40, 000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Type image 2	WB
Dilution image 2	1:500 - 1:2, 500
Immunogen	Rabbit IgG whole molecule
Concentration (mg/ml)	1.0 mg/mL by UV absorbance at 280 nm
Conjugate	Alkaline Phosphatase (Calf Intestine)
Application Note	ELISA 1:2, 000-1:10, 000; Western Blot 1:500-1:2, 500; Immunochemistry 1:200-1:1, 000
Buffer	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Target Species	Rabbit
Physical State	Liquid (sterile filtered)
Target Isotype	IgG (H&L)
Host Isotype	IgG
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide
Shipping Condition	Wet Ice
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins.
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 mg

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