HDAC2 Antibody (Center)

Q92769

IF, FC, IHC-P, WB

Ishikawa, F., et al. Oncogene 29(6):909-919(2010)
Bush, E.W., et al. Circ. Res. 106(2):272-284(2010)
Krishnan, M., et al. Oncogene 29(2):305-312(2010)
Lehmann, A., et al. BMC Cancer 9, 395 (2009)
Hassig, C.A., et al. Proc. Natl. Acad. Sci. U.S.A. 95(7):3519-3524(1998)

3066

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (Center)(Cat#AW5261).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HDAC2 immunoreactivity is localized to Nucleus significantly.

1:10~50

WB

HDAC2 Antibody (Center) (Cat. #AW5261) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HDAC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

1:50~100

FC

P70288; P56519

Rabbit Ig

410-439

This HDAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the Central region of human HDAC2.