Anti-Human GPR43/FFAR2 (extracellular)

Antibodies

Antibodies

Israel

Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)

Polyclonal

Western blot analysis

25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

1% BSA, 0.05% NaN3

FFAR2

Human

41116161

Free fatty acids (FFAs) are essential nutrients that are also involved in signal transduction through FFA receptors, members of the G-protein coupled receptor superfamily.FFAR2/GPR43 is an FFA receptor that is activated specifically by short fatty acids (acetate, propionate and butyrate), that are the products of bacterial fermentation in the gut1.GPR43 is expressed in adipose tissue, intestines, and immune tissues2 and its stimulation by interaction with short fatty acids inhibits cAMP production, activates the extracellular signal-regulated kinase (ERK) cascade via interactions with the Gi/o family of G-proteins. This increases intracellular Ca2+ levels, and promotes activation of the mitogen-activated protein kinase (MAPK) cascade through interactions with the Gq family of G-proteins1.These processes, mediated by GPR43 receptor regulate the host energy homeostasis and hence GPR43 is shown to be related to metabolic disorders, such as obesity and diabetes1.Because of its pivotal role in energy homeostasis and in regulating many cellular processes, GPR43 has many phenotypic effects: mutant mice are hyper susceptible to developing intestinal carcinogenesis3, Klebsiella pneumoniae infection4 and to increased differentiation of bone marrow cells into osteoclasts5. Studies have shown the role of GPR43 in regulating inflammatory responses, which can lead to colitis and asthma6, 7. The various activities mediated by GPR43 makes it an appealing therapeutic target.