Anti-rat p75

Antibodies

Israel

Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR

Monoclonal

Western blot analysis

10 μl, 50 μl, 100 μl or 250 μl double distilled water (DDW), depending on the sample size.

1% BSA, 0.05% NaN3

192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin.

7, 4

p75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for b-amyloid, and for the myelin axon repellant protein MAG.1, 2, 4, 6 Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1, 3, 6 Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain. p75NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity, and is more potently activated by pro-neurotrophins.5Overexpression of the intracellular domain in developing neurons induces apoptosis. NGF-dependent association of NRIF with p75NTR contributes to pro-apoptotic signaling.7, 8 Beta-amyloid peptide binds p75NTR and stimulates pro apoptotic signaling by p75NTR, suggesting that the receptor may be relevant for Alzheimer's disease.9