Anti-Na+/H+ Exchanger 2 (NHE-2)

Antibodies

Sodium/hydrogen exchanger 2, SLC9A2

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4

Sodium/hydrogen exchanger 2, SLC9A2 - A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 2 (NHE-2)

Mouse - identical; human - 12/16 amino acid residues identical

Confirmed by amino acid analysis and mass spectrometry

0.8 mg/ml

Intracellular, C-terminus

Rat

41116161

In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1.To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1, 3, 4.Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1, 5, 6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1, 5, 8.NHE-2 is detected in the intestine, kidney and parietal cells2, 9-11. It is also detected in skeletal muscle and testis2, 12.