Anti-K2P16.1 (TALK-1) (extracellular)

Antibodies

Israel

Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

A Rabbit Polyclonal Antibody to KCNK16 Channel

Polyclonal

Western blot analysis

25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

1% BSA, 0.05% NaN3

KCNK16

7, 4

yes

The KCNK16 gene encodes the TALK-1 channel, an abundant K+ channel transcript found in mouse and in human β-cells. There are four human TALK-1 transcript variants, including two that form functional K+ channels (TALK-1a (transcript variant 2) and TALK1-b (transcript variant 3)1, 2. The TALK-1 structure includes 4 transmembrane domains, 2 extracellular loops, and intracellular N- and C-termini. The C-terminal tail of TALK-1 is a critical component that influences the magnitude of TALK-1 channel activation, while the N-terminus is important for protein interaction1, 2.TALK-1, two-pore domain K+ (K2P16.1) channel is responsible for modulating glucose stimulated insulin secretion that relies on β-cell Ca2+ influx. The channel produces outwardly rectifying, non-inactivating K+ currents, which are enhanced by elevations in extracellular pH. TALK-1 is thus pH-sensitive, with increased activity under alkaline conditions. In addition, it can be activated by singlet oxygen and nitric oxide.A gain-of-function polymorphism in the KCNK16 gene is associated with increased risk for developing type-2 diabetes1, 2.