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Interleukin-8 (IL-8)

Hersteller Anogen
Kategorie
Typ Antibody Monoclonal
Specific against Human
Clon I8-60
Isotype IgG1 kappa
Format Aff. Pur
Applikationen WB, ELISA, NT
Protein Familie Cytokine, Growth Factors
Menge 0.5mg
Host Mouse
ArtNr ANOG-MO-C40017D
Targets CXCL8
eClass 6.1 32160702
eClass 9.0 42030590
Lieferbar
Description
Mouse monoclonal antibody to human interleukin 8 (IL-8)
Target Protein
Human IL-8
Immunogen
Purified recombinant human IL-8
Specificity
This antibody recognizes both recombinant and native human IL-8.
Purification
Protein G affinity purified
Species Reactivity
Human, other species not tested
Cross Reactivity
This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Myeloma
P3X63Ag8.653
Background
Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
Formulation
Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Application info
ELISA: This antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MO-C40017A).
Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.
Western Blot: Concentration of 0.02-0.1ug/mL antibody will allow visualization of 100ng/lane of human IL-8.
Research Area
Cytokine, interleukin and chemotaxis
Storage
Store at -20C

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.5mg
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 13.05.2024 

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