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Interleukin-8 (IL-8)

Interleukin-8 (IL-8)

Manufacturer	Anogen
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Antibodies By Cell Type Carcinoma Western Blot ELISA Primary Antibodies By Organ Heart & Blood Cancer Neutralization
Type	Antibody Monoclonal
Specific against	Human
Clone	I8-60
Isotype	IgG1 kappa
Format	Aff. Pur
Applications	WB, ELISA, NT
Protein Family	Cytokine, Growth Factors
Amount	0.5mg
Host	Mouse
Item no.	ANOG-MO-C40017D
Targets	CXCL8
eClass 6.1	32160702
eClass 9.0	42030590
Available
Description	Mouse monoclonal antibody to human interleukin 8 (IL-8)
Target Protein	Human IL-8
Immunogen	Purified recombinant human IL-8
Specificity	This antibody recognizes both recombinant and native human IL-8.
Purification	Protein G affinity purified
Species Reactivity	Human, other species not tested
Cross Reactivity	This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Myeloma	P3X63Ag8.653
Background	Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
Formulation	Lyophilized from a solution in 0.01M PBS, pH 7.0
Reconstitution	Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Application info	ELISA: This antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MO-C40017A). Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes. Western Blot: Concentration of 0.02-0.1ug/mL antibody will allow visualization of 100ng/lane of human IL-8.
Research Area	Cytokine, interleukin and chemotaxis
Storage	Store at -20C

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Amount: 0.5mg

available

Delivery expected until 6/17/2024