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CD47(B6H12.2), CF700 conjugate, 0.1mg/mL

ArtNr B-BNC000437-100
Hersteller Biotium
Menge 100 uL
Quantity options 100 uL 500 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, FC, IF, IP
Clon B6H12.2
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag CF700
Citations
1. J Biol Chem (2002) 277(12): 10028-10036. (functional studies)
2. Cell (2009) 138(2): 286-299. (functional studies)
3. J Immunol (2014) 193: 3914-3924. (immunofluorescence)
4. J Immunol (2015) 195: 661-671. (flow, surface; immunofluorescence; western)
5. Nat Med (2019) 25(4): 612-619. (immunoprecipitation)
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary
Manufacturer - Applications
IF (published)|Flow, surface (verified)|Functional studies (published)|IF (verified)|IP (published)|WB (published)
Manufacturer - Category
Antibodies
Manufacturer - Targets
CD47
Manufacturer - Conjugate / Tag
CF700
Manufacturer - Host
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature
Room temperature
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Molecular Weight
~50 kDa
Manufacturer - Research Area
Cell adhesion|Hematology|Neuroscience|Signal transduction
Description
This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.


Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
Intact CD47 purified from placenta
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
CD47
Unigene
446414
Positive Control
HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors|MCF-7
Antibody target cellular localization
Plasma membrane
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Expected Antibody Applications
Functional studies (published for clone)|IP (published for clone)|WB (published for clone)
Manufacturer - Antibody Reactivity
CD47

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 uL
Lieferbar: In stock
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