ArtNr |
B-BNC000437-100 |
Hersteller |
Biotium
|
Menge |
100 uL |
Quantity options |
100 uL
500 uL
|
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, FC, IF, IP |
Clon |
B6H12.2 |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus) |
Host |
Mouse |
Isotype |
IgG1 Kappa |
Konjugat/Tag |
CF700 |
Citations |
1. J Biol Chem (2002) 277(12): 10028-10036. (functional studies) 2. Cell (2009) 138(2): 286-299. (functional studies) 3. J Immunol (2014) 193: 3914-3924. (immunofluorescence) 4. J Immunol (2015) 195: 661-671. (flow, surface; immunofluorescence; western) 5. Nat Med (2019) 25(4): 612-619. (immunoprecipitation) |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen |
Versandbedingung |
Raumtemperatur |
Lieferbar |
|
Manufacturer - Type |
Primary |
Manufacturer - Applications |
IF (published)|Flow, surface (verified)|Functional studies (published)|IF (verified)|IP (published)|WB (published) |
Manufacturer - Category |
Antibodies |
Manufacturer - Targets |
CD47 |
Manufacturer - Conjugate / Tag |
CF700 |
Manufacturer - Host |
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells. |
Shipping Temperature |
Room temperature |
Storage Conditions |
Store at 2 to 8°C|Protect fluorescent conjugates from light |
Molecular Weight |
~50 kDa |
Manufacturer - Research Area |
Cell adhesion|Hematology|Neuroscience|Signal transduction |
Description |
This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
|
Product origin |
Animal |
Ingredient of biological origin |
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells. |
Special handling for PI and label |
Protect from light |
Undated stability guarantee in PI |
2 years |
Stability at RT during shipping (protected from light) |
Stable at room temperature or 37°C (98°F) for 7 days. |
UNSPSC Commodity |
41116161 |
UNSPSC Commodity Title |
Primary and secondary antibodies for multiple methodology immunostaining detection application |
Classified or regulated chemicals |
0.05% sodium azide (CAS 26628-22-8) |
Concentration |
0.1 mg/mL |
Storage buffer |
PBS, 0.1% BSA, 0.05% azide |
Dated shelf life printed on label |
Guaranteed for at least 24 months from date of receipt when stored as recommended |
Immunogen (Secondaries, for anti tag only) |
Intact CD47 purified from placenta |
Clonality |
Monoclonal |
Regulatory Status |
For research use only (RUO) |
Human Gene Symbol |
CD47 |
Unigene |
446414 |
Positive Control |
HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors|MCF-7 |
Antibody target cellular localization |
Plasma membrane |
Antibody application notes |
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user |
Expected Antibody Applications |
Functional studies (published for clone)|IP (published for clone)|WB (published for clone) |
Manufacturer - Antibody Reactivity |
CD47 |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.