PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) (PDL1/2746), CF543 conjugate, 0.1mg/mL

Flow, surface (verified)|IF (verified)|IHC, FFPE (verified)|WB (verified)

B7-H1|CD274|PD-L1|PDCD1LG1

Room temperature

37-50 kDa

PD-L1 (also known as Programmed Death Ligand 1, CD274, and B7-H1) is one of two cell surface ligands that engage the negative regulatory T cell surface receptor PD-1. PD-L1 is a Type I transmembrane protein expressed on the plasma membrane of cells, such as activated macrophages and some tumor cells. Because binding of PD-1 by PD-L1 has a suppressive effect on the immune system, inhibition of the PD-1/PD-L1 pathway is a major strategy of immune-oncology therapies. PD-L1 is upregulated in certain cancers, and blocking PD-1 or PD-L1 can inhibit cancer growth.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

2 years

41116161

0.05% sodium azide (CAS 26628-22-8)

PBS, 0.1% BSA, 0.05% azide

Recombinant fragment of human CD274 protein (around aa 39-191) (exact sequence is proprietary)

For research use only (RUO)

521989

Secreted (extracellular)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Western blotting 1-2 ug/mL|Flow Cytometry 1-2 ug/million cells in 0.1 mL|ELISA For coating, order Ab without BSA|Optimal dilution for a specific application should be determined by user

Flow (surface) (verified)|IF (verified)|IHC (FFPE) (verified)|WB (verified)