Cytokeratin 5/8(C-50), Biotin conjugate, 0.1mg/mL

IHC, FFPE (verified)

Cytokeratin 5/8

Room temperature

58 kDa (CK5); 52.5 kDa (CK8)

This antibody reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodology
immunostaining detection application

0.1 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

KRT5

HepG2 or A431 cells. Skin or Squamous cell carcinoma

Epithelial cells

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1.0 ug/mL|For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/mL PBS, 15 min at RT|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Western blotting 0.5-1.0 ug/mL|Does not react with chicken , or Xenopus|Optimal dilution for a specific application should be determined by user

Cytokeratin 5/8