CD15 / FUT4 / Lewis x(FR4A5), 0.2mg/mL

Primary

Primary Antibodies Only

Purified, with BSA

Room temperature

~220 kDa (Glycoprotein)

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on more than 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin'€™s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodology
immunostaining detection application

0.2 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

FUT4

U937 cells, Reed-Sternberg' s cells in Hodgkin' s lymphoma

Granulocytes

Flow (verified), IF (verified)

Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

CD15|FUT4|Lewis-x