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Anti-ALKBH5 Antibody Picoband® Europäischer Partner

ArtNr BOS-A03360
Hersteller Boster
Menge 100 ug/vial
Quantity options 100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Kategorie
Typ Antibody
Format Lyophilized
Applikationen WB, IF, ICC, ELISA
Specific against Human (Homo sapiens)
Host Rabbit
Isotype IgG
Citations 1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/12/2010.
2. Tsujikawa, K., Koike, K., Kitae, K., Shinkawa, A., Arima, H., Suzuki, T., Tsuchiya, M., Makino, Y., Furukawa, T., Konishi, N., Yamamoto, H. Expression and sub-cellular localization of human ABH family molecules. J. Cell. Molec. Med. 11: 1105-1116, 2007.
3. Zheng, G., Dahl, J. A., Niu, Y., Fedorcsak, P., Huang, C.-M., Li, C. J., Vagbo, C. B., Shi, Y., Wang, W.-L., Song, S.-H., Lu, Z., Bosmans, R. P. G., and 16 others. ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility. Molec. Cell 49: 18-29, 2013.
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias RNA demethylase ALKBH5,Alkylated DNA repair protein alkB homolog 5,Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5,ALKBH5,ABH5,OFOXD1
Lieferbar
Manufacturer - Category
Primary Antibodies
Storage Conditions
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight
52 kDa
Clonality
Polyclonal
Application Details
Western blot, 0.1-0.25μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
ELISA, 0.1-0.5μg/ml
Gene Name
ALKBH5
Gene Full Name
alkB homolog 5, RNA demethylase
Manufacturer - Specificity
No cross reactivity with other proteins.
Background
RNA demethylase ALKBH5 is an enzyme that in humans is encoded by the ALKBH5 gene. It is mapped to 17p11.2. N (6)-methyladenosine (m (6)A) is the most prevalent modification of mRNA. ALKBH5 functions as a demethylase that removes m (6)A from mRNAs.
Immunogen
E.coli-derived human ALKBH5 recombinant protein (Position: E81-R180).
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category
Adaptive Immunity, Immunology, Lipid Signaling, Signal Transduction, Signaling Pathway, T Cells
Protein Function
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N6-methyladenosine in single-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
Subcellular Localization
Nucleus speckle.
Description
Boster Bio Anti-ALKBH5 Antibody Picoband® catalog # A03360. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity
Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
Manufacturer - Cross Reactivity
No cross-reactivity with other proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug/vial
Lieferbar: In stock
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