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Bulk Anti-Human CD56 (ERIC-1) Antibody Europäischer Partner

ArtNr ICH1015-1MG
Hersteller IchorBio
Menge 1 mg
Quantity options 0.5 mg 100 mg 1 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody
Applikationen WB, IP, IHC, ELISA, Dot
Clon ERIC-1
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
Purity >95% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Neural cell adhesion molecule 1, NCAM1
Versandbedingung Gekühlt
Lieferbar
Specificity Anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) recognizes Human CD56. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM)
Manufacturer - Type
Low Endotoxin, anti-human
Manufacturer - Applications
ELISA Capture, IHC (Frozen), IHC (Paraffin), Western Blot, Immunoprecipitation, Dot Blot
Manufacturer - Category
Low Endotoxin, anti-human, monoclonal antibody
Manufacturer - Isotype
Mouse IgG1
Manufacturer - Targets
CD56
Shipping Temperature
Blue ice
Storage Conditions
anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 5%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20EC) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes.
Application Notes
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 80 ng/mL.
Dot Blots: Immunoblotting: Recognizes 120, 140, and 180 kDa NCAM isoforms.
Each investigator should determine their own optimal working dilution for specific applications.
Background
Anti-Human CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested
Concentration
1.0 - 5.0 mg/ml
Endotoxin
<0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) recognizes Human CD56. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM)
Purification method
This monoclonal antibody was purified using Protein A
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
Human Retinoblastoma tumor
Isotype Control
Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
Buffer
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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