ArtNr |
ICH1015-1MG |
Hersteller |
IchorBio
|
Menge |
1 mg |
Quantity options |
0.5 mg
100 mg
1 mg
25 mg
50 mg
5 mg
|
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, IP, IHC, ELISA, Dot |
Clon |
ERIC-1 |
Specific against |
Human (Homo sapiens) |
Host |
Mouse |
Isotype |
IgG1 |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Neural cell adhesion molecule 1, NCAM1 |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
Anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) recognizes Human CD56. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM) |
Manufacturer - Type |
Low Endotoxin, anti-human |
Manufacturer - Applications |
ELISA Capture, IHC (Frozen), IHC (Paraffin), Western Blot, Immunoprecipitation, Dot Blot |
Manufacturer - Category |
Low Endotoxin, anti-human, monoclonal antibody |
Manufacturer - Isotype |
Mouse IgG1 |
Manufacturer - Targets |
CD56 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20EC) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes. |
Application Notes |
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 80 ng/mL. Dot Blots: Immunoblotting: Recognizes 120, 140, and 180 kDa NCAM isoforms. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
Anti-Human CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested |
Concentration |
1.0 - 5.0 mg/ml |
Endotoxin |
<0.5 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) recognizes Human CD56. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM) |
Purification method |
This monoclonal antibody was purified using Protein A |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
Human Retinoblastoma tumor |
Isotype Control |
Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247) |
Buffer |
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.