Hersteller |
IchorBio
|
Kategorie |
|
Typ |
Antibody |
Specific against |
Mouse |
Clon |
2.4G2 |
Isotype |
IgG2b |
Applikationen |
WB, Blocking |
Menge |
1mg |
Host |
Rat |
ArtNr |
ICH1056-1MG |
Targets |
CD32 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Aggregation |
Aggregation level <= 5% |
Antigen Distribution |
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes. |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcgammaIII and FcgammaII, and possibly FcgammaI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo. |
Concentration |
0.5 mg/ml |
Endotoxin |
<= 1.0 EU/mg as determined by the LAL method |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Product Type |
Low Endotoxin, anti-mouse |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Storage |
anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8C. For long term storage aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.