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Antibodies

Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) Europäischer Partner

Hersteller IchorBio
Kategorie
Typ Antibody
Specific against Mouse
Clon 2.4G2
Isotype IgG2b
Applikationen WB, Blocking
Menge 1mg
Host Rat
ArtNr ICH1056-1MG
Targets CD32
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Aggregation
Aggregation level <= 5%
Antigen Distribution
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Background
CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcgammaIII and FcgammaII, and possibly FcgammaI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.
Concentration
0.5 mg/ml
Endotoxin
<= 1.0 EU/mg as determined by the LAL method
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Product Type
Low Endotoxin, anti-mouse
Purification method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Storage
anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8C. For long term storage aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles.
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1mg
Lieferbar: In stock
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Lieferung vsl. bis 02.05.2024 

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