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Anti-Mouse IL-4 (11B11) In Vivo Antibody - Ultra-Low Endotoxin Europäischer Partner

ArtNr ICH1087UL-5MG
Hersteller IchorBio
Menge 5 mg
Quantity options 100 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody
Applikationen FC, IP, IHC, ELISA, FA, NT
Clon 11B11
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG1 Kappa
Purity >98% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Interleukin-4, Il-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1
Versandbedingung Gekühlt
Lieferbar
Specificity Anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4
Manufacturer - Applications
ELISA Capture, ELISPOT, Flow Cytometry, Neutralization, Immunoprecipitation, Functional Assays, CyTOF, IHC
Manufacturer - Category
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG1 Kappa
Manufacturer - Targets
IL-4
Shipping Temperature
Blue ice
Storage Conditions
anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) is stable when stored at 2-8°C for 1 week. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 1%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Application Notes
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml.
ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 µg/ml is recommended.
Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo.
Each investigator should determine their own optimal working dilution for specific applications.
Background
Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.
Concentration
1.0 - 5.0 mg/ml
Endotoxin
≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-IL-4 In Vivo Antibody - Ultra-Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
Purified Recombinant Mouse IL-4 (>98%)
IMPACT Pathogen Test
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
Antibodies against the same target (Cat No.)
Isotype Control
Rat IgG1 Isotype Control for In Vivo - Low Endotoxin [GL113] (ICH2246)
Buffer
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5 mg
Lieferbar: In stock
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