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Anti-Mouse CD49d (PS/2) In Vivo Antibody - Ultra-Low Endotoxin Europäischer Partner

ArtNr ICH1200UL-50mg
Hersteller IchorBio
Menge 50 mg
Quantity options 100 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody Monoclonal
Applikationen FC, IP, IHC, FA
Clon PS/2
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2b Kappa
Purity >98% by SDS-PAGE and HPLC
Formula Sterile, preservative-free, solution in PBS. BSA and Azide free.
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias VLA-4α, ITGA4, Integrin α4
Versandbedingung Gekühlt
Lieferbar
Specificity PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.
Manufacturer - Applications
Functional Assays, Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Manufacturer - Category
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2b Kappa
Manufacturer - Targets
CD49d
Shipping Temperature
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 1%
Formulation
Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution
CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Background
Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture.
Concentration
≥ 2.0 mg/ml
Endotoxin
≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity
PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0.
IMPACT Pathogen Test
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
Isotype Control
Rat IgG2b In Vivo Isotype Control – Low Endotoxin (1-2) [ICH2243]
Buffer
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 mg
Lieferbar: In stock
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