Anti-Mouse CD183 (CXCR3-173) In Vivo Antibody - Low Endotoxin

Flow Cytometry, Neutralization

Armenian Hamster IgG

Blue ice

Aggregation level ≤ 5%

CXCL3 is expressed on primary memory phenotype CD4+ and CD8+ T cells, naturally occurring CD4+CD25+ Foxp3+ regulatory T cells, natural killer (NK) T cells, and approximately 25% of NK cells. CXCR3-173 recognizes an epitope of CXCR3 expressed on the surface of activated mouse splenocytes.

Hamsters were tested by ELISA for seropositivity against CXCR3 peptide and hybridomas were generated, screened by FACS, purified, and tested for: staining, CXCR3 blockade in vitro, and endotoxin levels. CXCR3-173 detects the native form of CXCR3, and therefore does not work in Western blotting.

CXCR3-173 has potential use in immunotherapeutic approaches to inhibit transplant rejection and immune related diseases. In vitro, CXCR3-173 blocks chemotaxis in response to CXCL10 or CXCL11 but not CXCL9. In vivo, CXCR3-173 prolongs both cardiac and islet allograft survival in a manner further enhanced by rapamycin.

<1.0 EU/mg as determined by the LAL method

This monoclonal antibody was purified using Protein A

CXCR3-173 was generated by immunizing Armenian hamsters with a peptide sequence unique to mouse CXCR3 which encompasses amino acids 1-37.

ICH3001-100ml; ICH3002-100ml; ICH3003-100ml