Anti-Mouse B7-H2 - 25 µg

Quality Tested by Leinco Western Blotting: To detect Mouse B7-H2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse B7-H2 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:B536.

B7-H2

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Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Human and mouse B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

Immunocytochemistry: This antibody was used at a concentration of 10 μg/ml to detect B7-H2 in Raw264 cells. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-goat IgG in the dark. Between each step, cells were washed with PBS containing BSA.

Goat Anti-Mouse B7 Homolog 2 (B7-H2) recognizes Mouse B7-H2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Purified Recombinant Mouse B7-H2 (> 98%)

Polyclonal Antibody, B7 Homolog 2, B7RP-1, B7h, LICOS, GL50, B7-H2, Mouse B7-H2, Anti-Mouse B7-H2, B7H2, B7-H2, B7H2B7 homolog 2, B7-H2B7-related protein 1, B7RP-1, B7RP1B7-like protein Gl50, B7RP-1LICOS, CD275 antigen, CD275, GL50, GL50transmembrane protein B7-H2 ICOS ligand, ICOSL, ICOS-L, ICOSLG, inducible T-cell co-stimulator ligand, KIAA0653ICOS ligand