Anti-Human BACE-1 - 25 µg

Quality Tested by Leinco Western Blotting: To detect Human BACE-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human BACE-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

BACE-1

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BACE-1 is an aspartic protease and an integral membrane protein. BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein β site in the brain to generate the amyloid β peptide. Because the amyloid β peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 -5.5), consistent with its proposed function in an acidic intracellular compartment.

Immunoprecipitation: Suitable for use at concentration of 25 µg/mL.

Goat Anti-Human Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE-1) recognizes Human BACE-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

NS0-Derived Recombinant Human BACE-1 Ectodomain

Polyclonal Antibody, Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1, BACE-1, Human Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1, Anti-Human BACE-1, APP beta-secretase, ASP2, Aspartyl protease 2, BACE1, BACE-1, BACEAsp 2, beta-secretase 1 precursor variant 1, beta-secretase 1, beta-site amyloid beta A4 precursor protein-cleaving enzyme, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, beta-site APP-cleaving enzyme 1, beta-site APP-cleaving enzyme EC 3.4.23, EC 3.4.23.46, FLJ90568, HSPC104, KIAA1149, memapsin-2, Membrane-associated aspartic protease 2, transmembrane aspartic proteinase Asp2