Anti-Human/Mouse BID - 100 µg

Quality Tested by Leinco Western Blotting: To detect Human/Mouse BID this polyclonal antibody can be used at a concentration of 1.0 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human/Mouse BID is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Simple Western Blotting: Recommended concentration 10 µg/mL.

BID

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Immunoprecipitation: Suitable for use at concentration of 4 µg/106 cells.

Goat Anti-Human/Mouse BID (BID) recognizes Human/Mouse BID. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.