Anti-Mouse CCL28 (Clone 134306) - 25 µg

Quality Tested by Leinco Western Blotting: To detect Mouse CCL28 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL28 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

CCL28

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CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.1, 2, 3 CCL28 is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.3 CCL28 is a key regulator of IgA antibody-secreting cells (ASCs) accumulation in the mammary gland and thus controls the passive transfer of IgA antibodies from mother to infant.4

Neutralization: This antibody is useful for neutralization of Mouse CCL28 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CCL28 (at 2 ng/ml) is 2.5 - 5 µg/ml.

Chemokine

AB_2828497

< 0.1 EU/µg as determined by the LAL method

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.