Anti-Mouse CD62L [Clone MEL-14] — DyLight® 488 - 200 µg

Quality Tested by Leinco FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

CD62L

Next Day 2 - 8°C

CD62L is a 75-95 kD glycoprotein which is also known as L-selectin, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells.

IP
B
IHC (Frozen)
IHC (Paraffin)
For specific conjugates of this clone, review literature for suggested application details.

Blue Laser (493 nm)

Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity

AB_2829176

This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.