Anti-Mouse CD8a (Ly 2) [53-6.7] (All Alleles) – PE - 50 µg

Quality Tested by Leinco FC The suggested concentration for this 53-6.7 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

CD8a

Next Day 2 - 8°C

CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

IHC (Frozen)
IHC (Paraffin)
IP
B
Depletion
For specific conjugates of this clone, review literature for suggested application details.

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Clone 53-6.7 recognizes Lyt 2.

0.2 mg/ml

Lyt 2 is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells.

Monoclonal Antibody, CD8a, Ly2, 53-6.72, Mouse, Anti-Mouse CD8a (Ly 2) (All Alleles)-R-phycoerythrin, cd8