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Anti-Mouse CD3ε (Clone 500A2) - Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD3ε (Clone 500A2) - Purified in vivo GOLD™ Functional Grade

ArtNr	LEIN-C219-50mg
Hersteller	Leinco Technologies
Menge	50 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Kategorie	In Vivo Antibodies In Vivo Antibodies
Typ	Antibody Monoclonal
Applikationen	FC, IHC, other, IV
Clon	500A2
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Syrian
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
NCBI	12501
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this 500A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD3ε
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Background	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Function	TCR signal transduction, T cell activation and antigen recognition.
Other Applications Reported In Literature	IHC (Frozen) The suggested concentration for this 500A2 antibody in IHC staining on frozen tissue is 2.5 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. B
PubMed	CD3ε
Ligand/Receptor	Peptide antigen/MHC-complex.
Manufacturer - Research Area	Cell Biology, Immunology
Manufacturer - Specificity	Clone 500A2 recognizes an epitope on mouse CD3ε.
RRID	AB_2737460
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD3ε is primarily expressed on mature T cells and NK-T cells and at different levels on differentiation-dependent thymocytes.
Immunogen	Mouse T-cell receptor
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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LEIN-C219-50mg-safety-datasheet.pdf