Anti-Mouse CD278 (ICOS) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Mouse ICOS cDNA and ICOS hexahistidine fusion protein

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2737463

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 7E.17G9 recognizes an epitope on mouse CD278.

Antigen Distribution

ICOS is expressed on activated T cells and APCs including splenic B cells, dendritic cells, and macrophages.

Background

CD278 (ICOS) belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers (47-57kD) and enhances all basic T cell responses to a foreign antigen, specifically proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. ICOS signaling is also an important regulator for T cell homeostasis. ICOS plays a role in humoral immune response and is thought to have involvement in the development of Th2 cells and to play a protective role in inflammatory autoimmune diseases.

Antigen Details

Protein

CD278

Ligand/Receptor

B7h/B7RP-1/GL-50

Function

Costimulates T cell activation, proliferation, humoral immune response

PubMed

CD278

NCBI Gene Bank ID

54167

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

1. Flavell, R. A. et al. (2001) Nature 409:97 2. Bachmann, M. F. et al. (2000) J. Exp. Med. 192:53 3. Kroczek, R. A. et al. (1999) Nature 397:263

Technical Protocols