Anti-Human DAN

Quality Tested by Leinco Western Blotting: To detect Human DAN this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human DAN is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

DAN

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DAN is a secreted glycoprotein related to Xenopus laevis cerberus. It is being referred to also as DAND1 [DAN domain family member 1]. The gene has been described also as NO3 gene, which shows tumor suppressor activity in cells transformed by the src oncogene.1 DAN belongs to a gene family, termed CAN family, that includes the head-inducing factor Gremlin, Cerberus, PRDC.2 DAN and several other members of the can family act as antagonists of BMP and appear to block BMP signaling by preventing bone morphogenetic proteins from interacting with their receptors. DAN has recently been demonstrated to have a tumor-suppressive activity in vitro and may have an important role in regulation of the entry of cells into the S phase.3

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Blocking: This antibody will block 95% of the binding of 100 ng/ml of Recombinant Human BMP-4 to immobilized Recombinant Human DAN (100 μl of 2 μg/ml was coated in each well) in a functional ELISA. 50% inhibition occurs at an antibody concentration of approximately 6 - 24 μg/ml.

Goat Anti-Human Differential Screening-Selected Gene Aberrative in Neuroblastoma (DAN) recognizes Human DAN. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

NS0-Derived Recombinant Human DAN