Anti-Human HGF R - 25 µg

B, CyTOF®, FC, ICC, IHC FFPE

HGF R

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Hepatocyte growth factor receptor (HGFR), also known as c-Met, plays an important roles in angiogenesis and tumor growth.1 C-Met is a receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC-γ. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis.2, 3

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.Blocking: Blockade of receptor-ligand interaction - Approximately 0.5-2 μg/ml of thisantibody will block 50% of the binding of 5 ng/ml of recombinant human HGF to immobilized recombinant human HGF R/Fc chimera (100 μl of a 1 μg/ml solution was coated in each well) in a functional ELISA.
Immunocytochemistry: Suitable for use at concentration of5-15 µg/mL.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Other Molecules

AB_2830317

< 0.1 EU/µg as determined by the LAL method

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.