Anti-Mouse IL-4 (Clone 11B11) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Partially Purified Native Mouse IL-4

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2830405

Applications and Recommended Usage?
Quality Tested by Leinco

ELISAThis antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 μg/ml. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

N
IHC
ICC
IP
CyTOF®
FA

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 11B11 recognizes an epitope on mouse IL-4.

Antigen Distribution

IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils.

Background

IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils^{1, 2}. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells^{3, 4} and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes⁵. In addition, IL-4 is implicated in normal wound healing^{6, 7} and airway inflammation in patients with asthma⁸.

Antigen Details

Protein

IL-4

PubMed

IL-4

NCBI Gene Bank ID

16189

UniProt.org

Information on Uniprot.org

Research Area

Immunology

References & Citations

1. Yoshimoto T & Paul WE. (1994) J Exp Med. 179(4):1285–95
2. Kipnis J., at al. (2012) J Immunol. 189(9):4213-4219
3. Groth B., et al. (1992) J Exp Med. 176:1091–8
4. Murphy KM., et al. (1992) Proc Natl Acad Sci USA. 89:6065–9
5. Paul WE., et al. (1999) Annual Rev Immunol. 17:701-38
6. Maquart FX., et al. (2000) Lab Invest. 80(8):1337-43
7. Godeau, G., et al. (2000) Lab Invest. 80, 1337–1343
8. Steinke JW & Borish L. (2001) Respir Res. 2(2):66-70

Technical Protocols